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In morphological study buy confido 60 caps line androgen hormone overdose, the plants were evergreen shrubs cheap confido 60caps with mastercard prostate oncology specialists inc, up to 3m tall; leaves simple, opposite and decussate, palmately 5-nerved; flowers showy pentamerous; Inflorescences corymbose cymes; Fruits were fleshy capsules and opening irregularly transversely at maturity. In histological study, the lower surface of the lamina were wavy, anomocyticrpe type of stomata were abundant in lower surface only. In transverse section of the lamina, the hypodermal layer was present below the epidermis. Scale-like multicellular shaggy hairs were occurred interface view of midrib and petiole. In transverse section of petiole, midrib and stem, intraxylary phloem was observed. Cortical and internal phloem was also found in transverse section of petioles and stems. In transverse section of old stem, sclereids and druses were prominently found in phelloderm layer. Transverse section of root, calcium oxalate (druses) were observed in phelloderm layer. Phytochemical screening, alkaloids were absent among the twelve tested constituents. Physicochemical properties showed that the powdered leaves were more soluble in polar solvents. Nutritional values of the leaves of the specimens were also studied because it is used for tonic and vegetables. The four organic compounds were isolated from the methanolic extracts of the specimens by applying column chromatography method. Based on the chemical test and spectroscopic data, isolated compounds A, B, C and D were may be assigned as β-sitosterol (steroid), ursolic acid, lupeol and α-amyrin (triterpenoid). The antimicrobial screening of the leaves and the whole plant extract by different solvents were tested against six pathogenic microorganisms using agar-well diffusion method. The methanolic extract from the leaves showed the highest activity especially more sensitive against Escherichia coli. In the case of the whole plant, all of the extracts (except aqueous) exhibited antimicrobial activity on all tested microbes, however; pet-ether (60°C-80°C) extract showed Bacillus subtilis only. Acute toxicity test of both aqueous and 70% ethanolic extracts from the whole plant of M malabathricum L. In antipyretic activity, both 70% ethanol and aqueous extracts of M malabathricum L. In this experiment, 70% ethanol and aqueous extracts showed significant reduction of fever on Wister albino rats. Cyanidin and quercetin from flowers were analysed by paper chromatographic and spectroscopic methods after acid hydrolysis. Flavonoids in leaves, mainly rutin, was extracted and the latter was isolated by paper chromatographic method and identified by chromatographic, chemical degradative and various spectroscopic methods. The yield percent of the chemical constituents in Nwe-thar-gi plant were cyaniding (1%) and quercetin 0. It was widely distributed and usually grown wild in marshy places, along roadsides and on banks of river and lakes of Myanmar, which was collected from Maubin Township, Ayeyarwady Division. The morphological and histological characters of the plant have been studied and identified by using available literature. In morphological study, the plant is annual or perennial herbs stem cylindrical, fine reticulate roots with tumid nodes and hollow. Leaves alternate and simple, superficial mucilage glands present on young leaves, ochreate stipule present. The histological characters of whole plant were studied, the cell walls of upper and lower surfaces of lamina are slightly wavy, and anomocytic stomata are present. The trichomes present are simple, tapering towards the ends with pointed tips and have a multicellular base sunken in the epidermis. Calcium oxalate crystals were present in mesophyll tissue of lamina, parenchymatous cells of midrib, petiole. Vascular bundles are collateral and close type form continuous ring, around the parenchymatous pith however in the petiole the vascular bundles are concentric. The preliminary phytochemical tests of the powdered sample of the whole plant were carried out.
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T he s h e l f - l i f e o f t h e r a d i o l i g a n d i s t h e r e b y i n c r e a s e d , an d s u f f i c i e n t a c t i v i t y i s p r e s e n t f o u r m o n th s a f t e r t h e a c t i v i t y d a t e t o a llo w p r e p a r a t io n o f s t a n d a r d c u r v e s w h ic h a r e s u p e r im p o s a b le u p on t h o s e p r e p a r e d w it h f r e s h t r a c e r. L o g - l o g i t l i n e a r r e g r e s s i o n a n a l y s i s o f t h e s t a n d a r d c u r v e d a t a g i v e s a c o r r e l a t i o n c o e f f i c i e n t e x c e e d in g 0. P r e c i s i o n o f t h e s t a n d a r d c u r v e i s e x c e l l e n t a t a l l f i x e d p o i n t s , c o e f f i c i e n t s o f v a r i a t i o n f o r s i x r e p l i c a t e s n o t e x c e e d in g 3. S e n s i t i v i t y o f t h e s ta n d a r d c u r v e , d e f i n e d a c c o r d in g t o K a is e r an d S p e c k e r [8 ] a s t h e l e a s t am ount d i s t i n g u i s h a b l e fro m z e r o a t t h e 95% c o n f id e n c e l e v e l , i s 4 p g p e r a s s a y tu b e ( 0. Comparison of oestriol concentrations measured in saliva dilutions (a) with (o) and without (*) a preliminary ether extraction, (b) with (°) and without (*) a preliminary hydrolysis with ß-glucuronidase. E x t r a c t io n o f u n c o n ju g a te d o e s t r i o l w it h d i e t h y l e t h e r i s u n n e c e s s a r y , h o w e v e r , s i n c e t h e o e s t r i o l c o n j u g a t e s , w h ic h c o n s t i t u t e t h e m a jo r f r a c t i o n o f p la sm a t o t a l o e s t r i o l , a r e n o t p r e s e n t i n s a l i v a i n s i g n i f i c a n t c o n c e n t r a t i o n s. M e a su r e m e n ts o f u n c o n j u g a t e d o e s t r i o l i n d i e t h y l e t h e r e x t r a c t s o f a s e r i e s o f d i l u t i o n s o f p o o le d s a l i v a s a m p le s sh o w ed n o s i g n i f i c a n t d e c r e a s e s i n m e a su r e d o e s t r i o l c o n c e n t r a t i o n s w hen co m p a red w it h t h o s e o b t a in e d u s in g t h e d i r e c t a s s a y. C o l i , from S ig m a , L on d on ) p r o d u c e d n o i n c r e a s e i n m e a su r e d o e s t r i o l c o n c e n t r a t i o n s ( F ig. T h is s i m p l e , d i r e c t a s s a y h a s now b e e n i n r e g u la r u s e f o r s i x m o n th s an d sh o w s i n t e r - an d i n t r a - a s s a y c o e f f i c i e n t s o f v a r i a t i o n o f l e s s th a n 9% i n a p p r o  p r i a t e lo w , m edium an d h ig h q u a l i t y c o n t r o l s. R e a g e n t s , a t t h e sam e c o n c e n  t r a t i o n s an d i n t h e sam e p r o p o r t io n s a s t h e m a n u a l a s s a y , a r e a s p i r a t e d , m ix e d an d pum ped th r o u g h a t h i r t e e n m in u te in c u b a t io n c o i l. S e p a r a t io n o f t h e b o u n d an d f r e e f r a c t i o n s t a k e s p l a c e b y m ean s o f a p o ly c a r b o n a t e m em b ran e. A s p i r a t i o n o f s a l i v a sa m p le o r s t a n d a r d f o r 60 s e c o n d s i s f o llo w e d b y 6 0 s e c o n d s o f w a s h , an d t h i s g i v e s a th r o u g h p u t r a t e o f 30 s a m p le s p e r h o u r w it h an a c c e p t a b l e sa m p le c a r r y o v e r o f <2%. F ig u r e 2 i l l u s t r a t e s d o s e r e s p o n s e c u r v e s an d p r e c i s o n p r o f i l e s o b t a in e d u s in g t h e sam e r e a g e n t s f o r t h e m a n u a l a s s a y an d t h e a u to m a te d a s s a y. A s e r i e s o f s i x s t a n d a r d c u r v e s w as g e n e r a t e d u s in g t h e a u t o  m a te d s y s te m an d co m p a red w it h s i x c u r v e s p r o d u c e d u s in g t h e m a n u a l a s s a y. M eans w e r e p l o t t e d f o r t h e r e s p o n s e m e ta m e te r (B /B 0 ) a t e a c h f i x e d p o i n t in t h e s t a n d a r d c u r v e s an d t h e c o e f f i c i e n t s o f v a r i a t i o n w e r e c a l c u l a t e d. T he a u to m a te d a s s a y sh o w s a lo w e r p o t e n  t i a l s e n s i t i v i t y (1 2 p g / t u b e , 0. S im ila r p r e c i s i o n p r o f i l e s w e r e o b t a in e d f o r t h e a u to m a te d an d m a n u a l a s s a y s. D e t e r m in a t io n s o f s a l i v a r y o e s t r i o l u s in g t h e a u to m a te d an d m an u a l m e th o d s sh ow a v e r y g o o d c o r r e l a t i o n ( r = 0. A lth o u g h t h e a u to m a te d s y s t e m h a s an a d e q u a te p e r fo r m a n c e i n i t s p r e s e n t fo r m , i t i s l i m i t e d i n s e n s i t i v i t y b y t h e r e q u ir e m e n t f o r c o u n t in g t h e u n b ou n d r a d i o l i g a n d f r a c t i o n. I t w as c o n s id e r e d a p p r o p r ia t e t o e s t a b l i s h : ( i ) t h e r e l a t i o n s h i p b e tw e e n p la s m a an d s a l i v a r y u n c o n ju g a te d o e s t r i o l c o n c e n t r a t i o n s , ( i i ) t h e s h o r t te r m ( h o u r ly ) a n d d a i l y v a r i a t i o n s in s a l i v a r y o e s t r i o l c o n c e n t r a t i o n s , ( i i i ) a p r o v i s i o n a l n o rm a l r a n g e f o r s a l i v a r y o e s t r i o l c o n c e n t r a t i o n s. S a l i v a r y o e s t r i o l c o n c e n t r a t i o n s w e r e d e te r m in e d b y t h e d i r e c t , s o l i d - p h a s e m e th o d. P la sm a a l i q u o t s (2 0 0 pL) w e r e e x t r a c t e d w it h d i e t h y l e t h e r (5 mL). E v a p o r a te d e x t r a c t s w e r e r e d i s s o l v e d i n a s s a y b u f f e r (1 mL) an d a l i q u o t s (2 0 0 yL) w e r e a s s a y e d a s d e s c r ib e d f o r s a l i v a s a m p le s. T h e s e r e s u l t s a r e i n g o o d a g r e e m e n t w it h t h o s e r e p o r t e d b y o t h e r w o r k e r s [ 2 , 1 0 ]. T he m ean i n d i v i d u a l v a r i a t i o n o v e r t h i s p e r io d w as 1 4.
